Automated Diagnostics of Neuroborreliosis Anti-Borrelia IgG Liquor – ORG 911GL Anti-Borrelia IgM Liquor – ORG 911ML ® 24 Alegria Test Strips The Alegria® Anti-Borrelia tests allow for quantitative automated detection of IgG or IgM class antibodies against Borrelia burgdorferi in human serum samples and in cerebrospinal fluid (CSF). According to current European guidelines, the diagnosis of neuroborreliosis is based on calculation of the specific anti-Borrelia antibody index and other clinical and laboratory findings. Currently, Alegria® is the only random access analyzer for rapid automated determination of the ratio of the specific antibody concentrations in CSF and a corresponding serum sample, which is necessary for calculation of the antibody index. The Anti-Borrelia kits from ORGENTEC Diagnostika use different sets of recombinant proteins displaying highly specific immunodominant epitopes of the major human pathogenic Borrelia species. 1) 2) 3) 4) Anti-Borrelia IgG Liquor: recombinant VlsE , DbpA , OspC and p83/p100 1) 3) 5) Anti-Borrelia IgM Liquor: recombinant VlsE , OspC and p41i 1. 2. 3. VlsE = VMP-like sequence expression site (VMP = variable major protein) DbpA = Decorin-binding-protein A OspC = Outer-surface-protein C 4. 5. p83/p100 = chromosomally encoded high molecular weight protein (HMW) p41i = 14 kD internal flagellin fragment These tailor-made antigen profiles cover the relevant epitopes for specific antibody recognition, improving sensitivity and specificity of the ORGENTEC Anti-Borrelia ELISAs compared to tests based on purified antigens from cultivated bacteria. CSF analysis with Alegria®− the benefit • First random access analyzer for automated CSF diagnostics • Cost-efficient and simple ELISA method for determination of the antibody index • Simultaneous measurement of multiple parameters for autoimmune and infectious disease serology in a single test run • Rapid automated analysis of up to 30 different tests in only 90 minutes • Each Alegria®Test Strip contains the complete set of reagents • Validation of each sample through individual internal control 1. Reiber H. External quality assessment in clinical neurochemistry: survey of analysis for cerebrospinal fluid (CSF) proteins based on CSF/serum quotients. Clin. Chem. 1995 41: 256. 2. Reiber H. and Peter J. B. Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. J. Neurol. Sci 2001. 184: 101. 3. Mygland A. et al. EFNS guidelines on the diagnosis and management of European Lyme neuroborreliosis. Eur. J. Neurol. 2010. 17: 8. 4. Huppertz H. I. et al. Rational diagnostic strategies for Lyme borreliosis in children and adolescents: recommendations by the Committee for Infectious Diseases and Vaccinations of the German Academy for Pediatrics and Adolescent Health. Eur. J. Pediatr. 2012. 171: 1619. 5. Djukic M. et al. Cerebrospinal fluid findings in adults with acute Lyme neuroborreliosis. J Neurol. 2012. 259: 630 05-2015 References:
