Эндогенный и экзогенный NO - Беломорская биологическая

NO:
:
:
.
2015
.
.
NO
NOS – NO-
Holmberg, A., Olsson, C., & Holmgren, S. (2006). The effects of
endogenous and exogenous nitric oxide on gut motility in
zebrafish Danio rerio embryos and larvae. The Journal of
Experimental Biology, 209(Pt 13), 2472–2479.
doi:10.1242/jeb.02272
:
1)
(nNOS)
2)
(iNOS)
3)
(eNOS)
NO
NOS – NO-
Holmberg, A., Olsson, C., & Holmgren, S. (2006). The effects of
endogenous and exogenous nitric oxide on gut motility in
zebrafish Danio rerio embryos and larvae. The Journal of
Experimental Biology, 209(Pt 13), 2472–2479.
doi:10.1242/jeb.02272
:
1)
(nNOS)
2)
(iNOS)
3)
(eNOS)
NO
NOS
BK
NO
K+
PKG
Ca2+
NO
NOS
BK
NO
K+
PKG
Ca2+
:
.
:
,
1)
2)
:
NO-
,
NO
NO
:
Gadus morhua
, 20-35
, N=20.
PowerLab
LabChart 7
:
1-2
1
).
,
=7,5
100
1
2
10
0
5
WASH
WASH
WASH
1
2
10
% = 100% ·
0
2/
1
5
WASH
WASH
WASH
L-NAME -
NO-
L-NAME
NOS
BK
NO
K+
PKG
Ca2+
L-NAME -
NO-
L-NAME
NOS
BK
NO
K+
PKG
Ca2+
NOS
L-NAME
SNP –
NO
NOS
SNP
NO
BK
NO
K+
PKG
SNP - sodium nitroprusside
Ca2+
SNP –
NO
NOS
SNP
NO
BK
NO
K+
PKG
SNP - sodium nitroprusside
Ca2+
NO
NO
?
NOS
BK
NO
NO
NO
K+
RhoK
NO?
NOS
NO
BK
K+
TEA (
)-
BKCa
L-NAME
NOS
NO
BK
K+
TEA
NOS
BKCa
1.
NOS
NO
2.
.
BKCa
.
NO
,
,
,
,
.
!