ЗАКОНОМЕРНОСТИ ДИНАМИКИ РОСТА КАЛЛУСА ЛИСТОВОГО

, 2009,
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40
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Abstract. The estimation of growth parameters of leaf origin callus of
«M.Sholokhov» kind lilac at cells cultivation on morphogene media in the presence of
exogenous phenol compounds was made with the help of adequate regression models
selection. On the basis of the analysis of models graphic presentation it has been
established that regressive models for all media turned out to be identical. The
reliability of differences in growth parameters was estimated on the 40-th day of
cultivation on the media of different composition. Maximum accumulation of raw
callus biomass, grown on the medium 2 was 80% higher, than that on the media 3 and
1
, 2009,
8,
1
4, and 64% higher than that on the medium 1. On the basis of step-by-step regressive
analysis it has been determined that media 3 and 4 are identical and are described by
one model equation. Thus, the medium, that provides high proliferative callus activity
is medium 2, containing rutin.
:
, 210023, .
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, 27,
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.37-09-29,
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[3-5].
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in vitro.
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[10,
8, 11].
in vitro
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(Syringa
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STATSOFT-RUSSIA
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“STATISTICA” 6.0 RUS.
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F,
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: 1 : F(11,48) = 18,747144, p = 0,0000;
: 2 : F(11,48) = 78,3964206, p = 00,0000;
: 3 : F(11,48) = 21,4078474, p = 0,0000;
: 4 : F(11,47) = 10,2708232, p = 0,000000003,
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Wt
t
G
µ
0,056679
0,027307
0,017883
0,009843
0,009570
0,123710 75,55619
0,256770 30,87506
0,392098 16,53741
0,712358 4,30707
0,732700 3,89137
0,000000
0,000000
0,000001
0,011639
0,017718
0,049434
0,026055
0,050776
0,144009
0,078830
0,950566
0,973945
0,949224
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3.
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100%
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1055,4
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2020,3
10764,4
Constant
-154,9
-661,4
t
4
517,1
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274588,8
127828,4
-127237,9
308,94
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148,42
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2).
2.
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.
1.
, . .
in vitro
/
2.
.
.–
, . .
.:
«
3.
/
.:
, 1999. – 160 .
(
)/
. .
.–
». – 2002. – 56 .
, .
.
/
.
.
.
. . .
//
.–
.:
, 1991. – . 5-20.
4. Plant Tissue Culture – A Potential Sourse of Medicinal Compounds /
S. R. Bhalsing [et al.] // J. Sci. Indust. Res. – 1998. – Vol. 57. – P. 703-708.
5.
.
in vitro /
. .
//
. – 1999. – . 46. – . 919-929.
6.
. .
,
. .
/
// 56
.–
12
.:
, 1996. – 45 .
, 2009,
8,
1
7. Ranen, P. H. Biology of plants / P. H. Ranen, R. F. Evert, S. E. Eichhorn. –
4-th ed. – Washington: Worth Publ., 1986. – . 351.
8. Role of cytoskeleton in cell shaping of developing mesophyll of weat /
G. Jung [et al.] // Eur. J. Cell Biol. – 1992. – Vol. 57. – P. 88-94.
9.
,
.
/
.
.
//
.
. –1996. – . 57. – . 293-325.
10. Ranen, P.H. Biology of plants / P.H. Ranen, R.F. Evert, S.E. Eichhorn. – 4-
th ed. – Washington: Worth Publ., 1986. – . 383.
11. Adventious root growth and cell cycle induction in deepwater rice /
R. Lorbiecke [et al.] // Plant Physiol. –1999. –Vol.119. – . 21-29.
12.
Syringa vulgaris L.
«
»
. .
[
in vitro /
.] //
13.
, .
.
14.
, .
. –2007. – . 6,
STATISTICA:
/ .
,
.–
.
.–
1. – . 105-113.
.:
, 2001. – 656 .
:
.:
, 1989. – 540 .
13
./
.